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Fig. 4 | Molecular Cancer

Fig. 4

From: Tumor-derived CXCL5 promotes human colorectal cancer metastasis through activation of the ERK/Elk-1/Snail and AKT/GSK3β/β-catenin pathways

Fig. 4

CXCL5/CXCR2 induces EMT in CRC cells via the ERK/Elk-1 pathway. a & b CXCL5 induces the phosphorylation of ERK and Akt in CRC cells in a CXCR2-dependent manner. No differences are observed in STAT3 and JNK pathway. c & d Representative immunofluorescence images showing that inhibition of the ERK pathway using U0126 upregulates E-cadherin expression and downregulates Vimentin expression in HCT116CXCL5 and SW480shRNA cells. Inhibition of the AKT pathway using LY294002 is not able to change expression of E-cadherin or Vimentin in HCT116CXCL5 and SW480shRNA cells. Scale, 50 μm. e & f Immunoblots showing that inhibition of the ERK pathway using U0126 upregulates E-cadherin expression and downregulates Vimentin expression in HCT116CXCL5 and SW480shRNA cells. Inhibition of the AKT pathway using LY294002 is not able to change the expression of E-cadherin or Vimentin in HCT116CXCL5 and SW480shRNA cells. Changes in Elk-1 are consistent with changes in ERK1/2

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Molecular Cancer

ISSN: 1476-4598