Fig. 5
MSLN knockdown reduces tumor formation and metastasis. NSG mice were injected subcutaneously with 1 × 106 cells of shMSLN or shC H2052 and H460 cells and analyzed for tumor formation and metastasis after 4 weeks. a Image of tumors from injection site of shC and shMSLN groups. b Tumor growth curves of local site tumor volume, means ± s.d. (n = 6), *P < 0.05 (c) Quantitation of local site tumor weights, means ± s.d. (n = 6), *P < 0.05. d Number of thoracic and abdominal surface metastatic nodules, means ± s.d. (n = 6), *P < 0.05. e H&E staining of pulmonary and hepatic tissue sections, arrows denote metastatic tumor nodules. f Immunohistochemistry staining of pulmonary and hepatic tissue sections with anti-human MSLN antibody, arrows denote human MSLN positive nodules. g Quantification of metastatic tumor areas, *P < 0.05