Fig. 3From: The depletion of PinX1 involved in the tumorigenesis of non-small cell lung cancer promotes cell proliferation via p15/cyclin D1 pathwayPinX1 suppresses cell proliferation and clonogenicity in NSCLC cell lines. a Western blotting analysis showed that the endogenous levels of PinX1 in four NSCLC cell lines (SK-MES-1, H125, A549, H1299) were lower than that in control normal lung tissues. NT, normal lung tissues. b PinX1 siRNA was introduced into two NSCLC cell lines, H1299 and A549. pCDH-PinX1 lentiviral were transuded into SK-MES-1. PinX1, Bcl-2, and Bax protein levels were assessed by using western blot analysis. c MTT assay was performed to measure viability of cells expressing different levels of PinX1. d Colony-forming assay was used to observe colony forming ability of cells expressing different levels of PinX1. Each bar represents the meanāĀ±āSD of three independent experiments. *, compared to control group (Pā<ā0.05)Back to article page