Fig. 6

Autophagy protects neuroblastoma cells from FOXO3-triggered apoptosis. a SH-EP/FOXO3 cells were transiently transfected with siCtr and siLC3 oligonucleotides and treated afterwards with 50 nM 4OHT for 6 h. LC3-I/LC3-II expression was determined by immunoblot analyses. GAPDH served as loading control. b SH-EP/FOXO3-siCtr and SH-EP/FOXO3-siLC3 cells were treated with 50 nM 4OHT for 24 h. PI-FACS analyses were performed to detect apoptotic cells. Shown are mean values ± s.e.m. of three independent experiments; statistical analysis was done with the Student’s unpaired t-test, **P < 0.025. c The caspase-3/7 activity assay was performed with SH-EP/FOXO3-siCtr and SH-EP/FOXO3-siLC3 cells treated with 50 nM 4OHT for 24 h. The caspase-3/7 activity was calculated between treated and untreated cells. Shown are mean values ± s.e.m. of three independent experiments; statistical analysis was done with the Student’s unpaired t-test, *P < 0.05