Fig. 6
ROS are induced after PLX4032 treatment of melanoma cells. a A375, 501 Mel or IGR37 cells were transiently transfected with plasmids encoding Grx1-roGFP2 or mito-roGFP2. Two days post transfection the cells were either subjected to a 1 h pretreatment with mitoQ (150 nM), a treatment with PLX4032 (3 h for A375; 10 h for 501Mel or (IGR37) or a combination thereof. Following this, the redox status was measured in the cytosol or mitochondria. Data were normalized to untreated cells, followed by one-way ANOVA coupled with Dunnett’s multiple comparisons tests. Representative graphs of two biological replicates are shown. b Western blot analysis of A375, IGR37 and 501Mel pre-treated for 1 h with mitoQ (150 nM) followed by 24 h of 1 μM PLX4032 treatment. Results are shown for one representative of three biological replicates. c Western blot analysis of A375, IGR37 and 501 Mel treated with hydrogen peroxide (H2O2; 100 μM) for the indicated time points. Results are shown for one representative of three biological replicates. *p > 0.05, **p > 0.01, ***p > 0.001