Fig. 8

Combination of AZD7545 and PLX4032 more efficiently suppresses melanoma growth compared to each compound alone. a Represenative experiment of A375 melanoma cells expressing iRFP treated either with 1 μM of PLX4032 or with 1 μM of PLX4032 in combination with 10 μM AZD7545 for 3 weeks. The intensity of red fluorescence was quantified and the bar diagram represents three biological replicates with their standard deviation. b Spheroid cultures of A375 melanoma cells were treated with DMSO control, with 1 μM of PLX4032 or with 1 μM of PLX4032 in combination with 10 μM AZD7545. After 3 days sphere diameters were measured and represented as bar diagrams. Error bars represent the standard deviation of a minimum of four technical replicates of one representative experiment of three biological replicates. c Twenty-four hours after plating, BRAFi-resistant A375 melanoma cell (A375-R) were stimulated with 10 μM of AZD7545. The plates were imaged using an IncuCyte ZOOM live cell microscope (Essen BioScience) and images were taken every 3 h for a total of 90 h. Results are shown for one representative of three biological replicates. Statistical significance was determined using paired Student’s t-tests. *p > 0.05, **p > 0.01, ***p > 0.001