Fig. 2

Silencing miR-210-3p inhibits bone metastasis of PC-3 cells in vivo. a Representative BLIs signal of bone metastasis of a mouse from the indicated groups of mice at 5 mins and 45 day respectively. b Representative radiographic images of bone metastases in the indicated mice (arrows indicate osteolytic lesions). c Representative H&E-stained sections of tibias from the indicated mouse (T, tumor; N, the adjacent non-tumor tissues). d The sum of bone metastasis scores for each mouse in tumor-bearing mice inoculated with vector (n = 9) or anti-miR-210-3p (n = 8) cells.​ *P < 0.05. e Quantification of the BLI signaling in the vector and miR-210-3p-downexpression groups at 45, 52 and 59 day respectively. *P < 0.05. f Kaplan-Meyer analysis of mouse survival in the vector and miR-210-3p-downexpression groups. g Kaplan-Meier analysis of mouse bone metastasis-free survival in the vector and miR-210-3p-downexpression groups. h Silencing miR-210-3p had no obvious effect on proliferation ability of PC-3 cells as assessed by MTT assay of proliferation (n=3). n.s. means no significance. i Silencing miR-210-3p converted a stick-like or long spindleshaped mesenchymal profile to a cobblestone-like or a short spindle-shaped epithelial morphology in PC-3 cells. j Silencing miR-210-3p increased E-cadherin expression and decreased Vimentin and Fibronectin expression in PC-3 cells. α-Tubulin served as the loading control. k Silencing miR-210-3p suppressed invasion and migration abilities in PC-3 cells. Error bars represent the mean ± S.D. of three independent experiments. *P < 0.05