Fig. 5

Hif-2α promote tumor progression in mouse models. a SW 1990 cells transfected with pcDNA3 or hif-2α (A530T) were injected subcutaneously as pairs in mice. The xenografts were much bigger when hif-2α (A530T) was transfected. ***, P < 0.001. b Nc-siRNA or sihif-2α was transfected to SW 1990 cells and a similar mouse model with a was generated. The xenografts with sihif-2α weighted lighter than nc-siRNA controls. **, P < 0.01. Dash lines indicate averages of tumor weight. c A similar mouse model with b was performed for PANC-1 cells instead of SW 1990 cells. Hif-2α knockdown inhibited xenograft growth. *, P < 0.05. Dash lines indicate averages of tumor weight. d Sixteen xenografts from mice in a and b were harvested and immunochemistry was performed for several proteins. Expression of vimentin, ZEB-1, Ki-67, and CD31 was positively correlated with hif-2α, while E-cadherin expression was negatively correlated with hif-2α. Bar, 110 μm. e Correlation analyses of expression between hif-2α and hif-1α, E-cadherin, vimentin, ZEB-1, Ki67, and CD31