Fig. 1

circMET overexpression is negatively related to poor survival in HCC patients a. circRNA expression in chr. 7q21-7q31 was determined by qRT-PCR in four pairs of HCC and matched adjacent nontumor liver tissues; b. Schematic illustration of the circularization of MET exon 2 forming circMET; c. The RNA expression of circMET was determined in 90 pairs of HCC and matched adjacent nontumor liver tissues; d. The expression of circMET was determined by in situ hybridization in 209 pairs of HCC and matched adjacent nontumor liver tissues (bar = 200 nm); e. The scatter diagram shows the expression of circMET determined by in situ hybridization in 209 pairs of HCC and matched adjacent nontumor liver tissues; f. circMET expression, analyzed by in situ hybridization in 209 HCC patients, is related to the overall survival and cumulative recurrence of HCC patients; g. The expression of MET was determined by qRT-PCR in 90 pairs of HCC and matched adjacent nontumor liver tissues; h. The expression of MET and circMET was determined by immunohistochemistry and in situ hybridization in 209 pairs of HCC and matched adjacent nontumor liver tissues (bar = 200 nm); i. No association was found between MET and circMET levels, as determined by qRT-PCR in 90 HCC tissues; j. A slight association was found between MET and circMET levels, as determined by IHC and in situ hybridization in HCC samples