Fig. 3

SOST promotes bone metastasis through the TGF-β/SMAD3 signaling. A Heatmap displayed the DEGs between the WT and SOST-silencing SCP2 cells after RNA-seq. B KEGG pathway enrichment of DEGs after SOST knockdown in SCP2 cells. C qRT-PCR analysis of the relative levels of SOST, KRAS, and TGFB mRNA transcripts in WT and SOST-silencing SCP2 cells or WT and SOST-over-expressing MDA-MB-231 cells. D A schematic diagram of the potential STAT3 binding sites in the TGFB and KRAS promoters predicted by rVista 2.0 software. E Western blot measurement of the relative levels of SOST, KRAS, TGF-β, SMAD3, CXCR4, and STAT3 to β-actin expression, and STAT3 phosphorylation in the indicated SCP2 cells or MDA-MB-231 cells