Fig. 6
From: CircZBTB44 promotes renal carcinoma progression by stabilizing HK3 mRNA structure
HK3 promoted RCC growth and metastasisin vitro and in vivo. (A) qRT-PCR and western blot analyses were used to detect the transfection efficacy of HK3 siRNAs (si-HK3-1/-2/-3) in RCC cells. (B) CCK-8 and (C) EdU assays were conducted to detect the impact of HK3 knockdown on RCC cell viability and proliferation. (D) Transwell and (E) Wound healing assays were used to detect the effects of HK3 knockdown on RCC cell migration. (F-G) The expression of HK3 in mouse tumor tissues was measured using qRT-PCR and western blot analyses. (H) Mouse tumor weight and (I) volume in indicated groups. (J) IHC assays were conducted to examine the Ki67 protein expression in mouse tumor tissues of indicated groups. ***P < 0.001