Fig. 8

The critical role of SLU7 in TNBC. (A) IB of SLU7 in MCF-10 A, MDA-MB-231, and MDA-MB-468. (B) IB of SLU7 in TNBC and normal tissue. (C) The correlation analysis between SLU7 and circCAPG in TNBC tissue. n = 132. (D) Cell proliferation assay of TNBC stable cell lines expressing control vector (Vector), SLU7 followed with infection of indicated control or circCAPG (OE-circCAPG). (scale bars, 1 cm). (E) Immunofluorescence with KI67 antibody in TNBC stable cell lines expressing control vector (Vector), SLU7 followed with infection of indicated control or circCAPG (scale bars, 50 μm). (F) Colony formation assay in TNBC stable cell lines expressing control vector (Vector), SLU7 followed with infection of indicated control or circCAPG. (scale bars, 1 cm) (G) IB of Cyclin D1 and CDK4 in TNBC stable cell lines expressing control vector (Vector), SLU7 followed with infection of indicated control or circCAPG. (H) Transwell assay of TNBC stable cell lines expressing control vector (Vector), SLU7 followed with infection of indicated control or circCAPG. (scale bars, 50 μm). (I) Wound healing assay of TNBC stable cell lines expressing control vector (Vector), SLU7 followed with infection of indicated control or circCAPG. (scale bars, 50 μm). (J) Image of TNBC patient-derived organoids infected with lentivirus expressing control or SLU7. All data were representative of at least three biological replicates and shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001