Fig. 3

Unique pathological features of malignancies in B^VETrp53^KO or B^VEpTEN^KO mice. A Pathological alterations in tissues or organs of HSC^VE, B^VETrp53^KO or B^VEpTEN^KO mice. Histological analysis of tissues or organs was carried out as described in Materials and Methods. Scale = 100 μm. B The disruption of splenic architecture in HSC^VE, B^VEP53^−/− and B^VEPTEN^−/− mice. Frozen spleen sections were stained with anti-B220 AF488 (green) for B cell zones, anti-CD3 AF549 (red) for T cell zones, and Hoechst for cellular nuclei as stated in Materials and Methods. Scale = 100 μm. C Fibrosis of bone marrow and spleen was induced by leukemic cells strongly in B^VEP53^−/− and B^VEPTEN^−/− mice, but weakly in HSC^VE mice. The bone marrow and spleen sections were stained with Picro Sirius Red for collagen. Scale = 100 μm. D NFκB signaling was activated in the spleen of B^VEP53^−/− mice but not other mice. The spleen sections were stained with anti-NFκB (active units) antibody as in Materials and Methods, and the positive signal was shown as brown color. Scale = 50 μm. All tissues were harvested from mice with disease at terminal stage or mice without disease at 28 weeks or indicated age. All images are representative of at least five mice per group and three independent experiments