Fig. 7

Transcription factors and regulators of germinal center reaction and memory B cells versus plasma cells differentiation are severely suppressed in splenic B^VETrp53^KO or B^VEpTEN^KO leukemic cells. A Expression of transcription factors/regulators that are involved in germinal center reaction and lineage differentiation of memory B cells versus plasma cells was altered in HSC^VE, B^VEP53^−/−, and B^VEPTEN^−/− leukemic cells. Expression data for 20 transcription factors/regulators was extracted from the whole RNA-seq data sets of splenic B cells or leukemic cells from wild type, B^VE, HSC^VE, B^VEP53^−/−, B^VEPTEN^−/− and B^VEP27^−/− mice, and a hierarchical clustering heatmap was generated as in Materials and Methods. B-D Quantitative PCR validation of expression of transcription factors/regulators for germinal center reaction (B), memory B cell commitment (C), and plasma cell commitment (D). In all experiments, splenic B cell RNAs were isolated from mice with terminal stage disease or mice without disease at 28 weeks. All data in B-D are representative of at least five mice per group and three independent experiments. **p < 0.01, ****p < 0.0001; n.s., not significant