Fig. 9
EVs-circEHD2 promotes the metastasis of RCC by regulating fibroblast activation. A, Representative images of α-SMA in RCC tissues, measured by IHC, according to the metastasis status. Scale bars: 50 μm. B, Representative images of MRC5 cells after incubation with PKH26-labeled RCC-EVs. Scale bars: 10 μm. C, Western blot showing the level of α-SMA in MRC5 cells after incubation with RCC-RVs. D, The ELISA results of IL-6 in MRC5 cells after incubation with RCC-EVs. E-G, Representative images of MRC5 cells cultured with PBS, OSRC-2-EVs and 786-O-EVs. Scale bars: 200 μm. (F) The collagen contraction and (G) the number of migrated cells. H-J, Representative images of in vivo bioluminescence imaging (H), gross appearance of the lung (I, left), hematoxylin and eosin staining (HE) (I, right), and quantification of lung metastatic colonization (J) in the tail vein of lung metastasis model treated with PBS (n = 3/group), Renca-EVsVector (n = 3/group), and Renca-EVscircEHD2 (n = 3/group). Error bars represent the standard deviation (SD) of three independent experiments. **, P < 0.01; ***, P < 0.001