Fig. 1

Identification of circ-YAP in CRC liver metastasis tissues. A, B. qRT-PCR analysis of circ-YAP expression in CRC tissues and cell lines. C. Sanger sequencing verifying the junction site of circ-YAP. D, E. Cells were treated with 3U/μg of RNase R or 5 μg/ml Actinomycin D, followed by qRT-PCR analysis of circ-YAP and YAP mRNA levels. F. FISH assay detecting the location of circ-YAP, DAPI was used to stain cell nucleus. Scale bar, 25 μm. G-I. ISH staining detecting circ-YAP expression in paraffin embedded tissues (G, H), followed by ROC curve analysis of the predictive accuracy (I). The dark purple denotes positive staining of circ-YAP. Scale bar, 50 μm. J. The survival curve of CRC patients with low and high circ-YAP levels. ***P < 0.001. Data (B, D, E) are the mean ± SD of three independent experiments carried out in triplicate