Skip to main content
Fig. 2 | Molecular Cancer

Fig. 2

From: A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis

Fig. 2

Silencing of circ-YAP alleviates CRC liver metastasis burden. A. qRT-PCR verifying the knockdown efficiency of the designed sgRNAs. B, C. Wound healing assay testing cell migration in circ-YAP-silenced LoVo cells. D, E. Transwell assay testing cell invasion in circ-YAP-silenced CRC cells. Scale bar, 100 μm. F. qRT-PCR verifying the overexpression efficiency of circ-YAP. G, H. Cell migration and invasion were tested in DLD1 and SW480 cells after circ-YAP overexpression. I. The sketch showing the establishment of the spontaneous liver metastasis model. J. The representative images of CRC liver metastasis in the indicated groups. Scale bar, 100 μm. K. The incidence of CRC liver metastasis in each group. ***P < 0.001. Data (A, C, E, F, G, H) are the mean ± SD of three independent experiments carried out in triplicate

Back to article page

Molecular Cancer

ISSN: 1476-4598