Fig. 5
YAP-220aa increases YAP activity. A-C. Co-IP assay testing the binding of YAP-220aa to LATS1. D, E. Co-IP assay testing the interaction between YAP and LATS1/14-3-3 after circ-YAP knockdown. F, G. Western blot analysis of the indicated protein levels in circ-YAP-silenced LoVo and SW620 cells transfected with circ-YAP or circ-YAP-m6A-mutation. H, I. IF staining and western blot testing the location of YAP in circ-YAP-overexpressing cells. Scale bar, 25 μm. J. Luciferase reporter assay testing the effect of circ-YAP knockdown on YAP transcription activity in CRC cells. K. qRT-PCR analysis of the pro-metastasis gene expression downstream of YAP after circ-YAP knockdown. L, M. Cell migration and invasion in HT29 and DLD1 cells after YAP knockdown or verteporfin treatment. N. The representative images and burden of spontaneous CRC liver metastases (n = 5 per group). Scale bar, 100 μm. ***P < 0.001. Data (J, L, M) are the mean ± SD of three independent experiments carried out in triplicate. The uncropped western blot data are provided as a Original Blot Image file