Fig. 3

ERK is activated following IFNγ treatment. (A) M230 and (B) M238 cells were serum starved for 48 h, followed by IFNγ treatment (100 U/ml) for up to 6 h. The resulting samples were probed with antibodies indicated to the right. Results are representative of 3 independent experiments per line. (C) M230 and (D) M238 cells were treated with IFNγ (100 U/ml) for the indicated time and probed with various antibodies as labeled to the right. Results are representative of 2 and 3 independent experiments for the M230 and M238 lines, respectively. See also Figs S4-S7 for images of full blots for each experiment shown in this figure