Fig. 3
From: ELF5 drives angiogenesis suppression though stabilizing WDTC1 in renal cell carcinoma
ELF5 acts as an angiogenesis inhibitor in RCC. (A) ENCORI database showed the correlation analysis of ELF5 and VEGFA expression. (B) Cell migration was determined by transwell assay after overexpressing ELF5 / ELF5-ΔSET. (C) Tube formation assay was employed for testing angiogenesis of HUVECs stimulated by the conditioned medium collected from the transfected 769-P and A498 cells. (D) ELISA results of VEGFA concentration in the conditioned medium of transfected 769-P and A498 cells. (E-F) RT-qPCR and western blot outcomes of VEGFA level in different cells. Two-tailed Student’s t test was performed. The error bars represent SD. ***p < 0.001