Fig. 6
From: ELF5 drives angiogenesis suppression though stabilizing WDTC1 in renal cell carcinoma
ELF5 transcriptionally activates USP3. (A) The hTFtarget database was employed for predicting target genes for ELF5. (B-C) UALCAN database was employed for predicting the expression of candidate target genes in KIRC. (D) The effect of ELF5 overexpression on the expression of the candidate genes was detected by RT-qPCR in 769-P cells. (E-F) RT-qPCR and western blot results of USP3 levels in cells transfected with pcDNA3.1-NC or pcDNA3.1-ELF5. (G) ChIP assay was utilized for determining the interaction of ELF5 and USP3 promoter. (H) The binding sites of ELF5 on USP3 promoter. (I) Luciferase reporter assay was carried out for validating the binding of ELF5 on USP3 promoter. Two-tailed Student’s t test was performed. The error bars represent SD. *p < 0.05, **p < 0.01, and ***p < 0.001