Fig. 7
From: ELF5 drives angiogenesis suppression though stabilizing WDTC1 in renal cell carcinoma
ELF5 enhances USP3-mediated WDTC1 stabilization. (A) BioGRID, HitPredict, MIST and Genemania database were employed for predicting the reciprocal protein of USP3. (B) UALCAN database was utilized to predict WDTC1 expression in KIRC. (C) Co-IP assay was implemented for detecting the endogenous interplay between USP3 and WDTC1. (D) GST pull-down assay was utilized to verify the direct interaction between USP3 and WDTC1. (E) Western blot results of WDTC1 level when USP3 was overexpressed in cells. (F) Western blot results of WDTC1 and USP3 levels in cells when ELF5 was overexpressed in cells. (G) Ubiquitination assay was implemented to test the impact of USP3 on WDTC1 ubiquitination level. (H-I) Western blot was implemented to determine WDTC1 level in CHX-treated cells when USP3 was overexpressed. Two-tailed Student’s t test was performed. The error bars represent SD. ***p < 0.001