Fig. 8

ELF5 inhibits RCC progression by upregulating WDTC1. (A) RT-qPCR result of WDTC1 expression in HK-2, 769-P, and A498 cells. (B) Colony formation assay was employed for detecting cell proliferative capability when WDTC1 was overexpressed. (C-E) Transwell assay was carried out for testing cell migratory and invasive capabilities. (F-G) Tube formation assay was employed for testing angiogenesis of HUVECs. (H-I) RT-qPCR and western blot outcomes of VEGFA level.(J) Tumor weight and the tumor growth curve were measured in WDTC1 overexpression cell and its control group (K-P) Cell proliferation (k), migration (l), invasion (m), angiogenesis (n), and VEGFA expression (o-p) were detected by colony formation assay (k), transwell assay (l-m), tube formation assay (n), RT-qPCR (o), and western blot (p) in cells of the pcDNA3.1 group, the pcDNA3.1-ELF5 group, the pcDNA3.1-ELF5 + sh-NC group, and the pcDNA3.1-ELF5 + sh-WDTC1 group. Two-tailed Student’s t test was performed. The error bars represent SD. *p < 0.05, **p < 0.01, and ***p < 0.001