Fig. 1
From: Ultra-sensitive CTC-based liquid biopsy for pancreatic cancer enabled by large blood volume analysis
Study overview and DLA findings: A Experimental setup (created in part with BioRender.com); B MNCs per mL in peripheral blood samples (D-PB, n = 60) versus matched DLA samples (D-DLA, n = 60); C Collection efficacy achieved for the targeted MNC population (n = 60). The filled circles indicate CTC-positivity (≥ 1 CTC detected); D CTC-concentration per mL in PB samples (light grey circles) and matched DLA samples (dark grey circles); E. Extrapolation of log-logistic fit curves for peripheral blood samples (PB) and DLA samples (DLA). Stair plots show the empirical CDF and the continuous line the fit. Extrapolation indicates probability (Cumulative Distribution) for a certain analyzed sample volume to have at least 1 CTC or 3 CTCs in every sample; F Genomic analysis of individual CS-CTCs isolated from DLA products with panel sequencing of KRAS and TP53 genes (T: tumor tissue); G Plots of copy number profiles along the 22 autosomes expressed as log2 ratios. Significant copy number gains and losses are highlighted in red and blue, respectively (M: FFPE material from metastatic tissue)