Fig. 2

Hsa_circ_0136666 promotes tumor proliferation and tumor immune escape. a Expression of hsa_circ_0136666 in various gastric cancer cell lines, compared to GES-1. b The effect of the overexpression of EIF4A3 on the expression abundance of hsa_circ_0136666. c The effect of the knockdown of EIF4A3 on the expression abundance of hsa_circ_0136666. d Overexpression of hsa_circ_0136666 promoted cell proliferation in MKN-45 cells. e Overexpression of hsa_circ_0136666 promoted cell proliferation in AGS cells. f Knockdown of hsa_circ_0136666 inhibited cell proliferation in MKN-45 cells. g Knockdown of hsa_circ_0136666 inhibited cell proliferation in AGS cells. h Overexpression of EIF4A3 promoted cell proliferation in MKN-45 cells. i Knockdown of EIF4A3 inhibited cell proliferation in MKN-45 cells. j Image of survival rate of overexpressing hsa_circ_0136666 tumor cells co-cultured with CD8⁺ T cells, n = 6. k Image of survival rate of knockdown hsa_circ_0136666 tumor cells co-cultured with CD8⁺ T cells, n = 6. l Western blot was used to detect immune checkpoint proteins and PI3 signaling pathway proteins in MKN-45 cells. m Western blot was used to detect immune checkpoint proteins and PI3 signaling pathway proteins in AGS cells. Data are presented as the mean ± SD, n = 3 if not mentioned. Student's t-test was used. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001