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Fig. 3 | Molecular Cancer

Fig. 3

From: hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma

Fig. 3

hsa_circ_0007919 inhibits DNA damage of GEM-resistant PDAC cells and proliferation of tumor in vivo

(A) Comet analysis of the DNA damage of GEM-resistant PDAC cells with or without hsa_circ_0007919 inhibition and of the DNA damage of PDAC cells with or without hsa_circ_0007919 overexpression under GEM treatment condition (200×). (B) IF analysis of γ-H2AX accumulation in GEM-resistant PDAC cells with or without hsa_circ_0007919 inhibition and of γ-H2AX accumulation in PDAC cells with or without hsa_circ_0007919 overexpression under GEM treatment condition (1000×). (C) Image of tumors formed by GEM-resistant PDAC cells with or without hsa_circ_0007919 inhibition (n = 5). (D-E) Growth curves of tumors formed by GEM-resistant PDAC cells with or without hsa_circ_0007919 inhibition (n = 5). (F-G) Weights of tumors formed by GEM-resistant PDAC cells with or without hsa_circ_0007919 inhibition (n = 5). (H) IHC analysis of the expression of LIG1, Ki67, caspase 3 and γ-H2AX in tumors formed by GEM-resistant PDAC cells with or without hsa_circ_0007919 inhibition (400×). (I-J) IHC staining score analysis of images from Fig. 3H. Data are the means ± SDs (n = 3 independent experiments), ** p < 0.01

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Molecular Cancer

ISSN: 1476-4598