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Fig. 5 | Molecular Cancer

Fig. 5

From: hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma

Fig. 5

LIG1 reversed cell proliferation, apoptosis and DNA damage effects of hsa_circ_0007919

(A-B) CCK-8 analysis of the proliferation of GEM-resistant PDAC cells with or without LIG1 inhibition. (C) Flow cytometry analysis of the apoptotic rate of PDAC-resistant PDAC cells with or without LIG1 inhibition. (D-E) Expression of apoptosis-related proteins in GEM-resistant PDAC cells with or without LIG1 inhibition. (F) Comet analysis of the DNA damage of GEM-resistant PDAC cells with or without LIG1 inhibition (200×). (G) IF analysis of γ-H2AX accumulation in GEM-resistant PDAC cells with or without LIG1 inhibition (1000×). (H-I) CCK-8 analysis of the proliferation of hsa_circ_0007919-inhibited GEM-resistant PDAC cells with or without LIG1 overexpression. (J) Flow cytometry analysis of the apoptotic rate of hsa_circ_0007919-inhibited PDAC-resistant PDAC cells with or without LIG1 overexpression. (K-L) Expression of apoptosis-related proteins in hsa_circ_0007919-inhibited GEM-resistant PDAC cells with or without LIG1 overexpression. (M) Comet analysis of the DNA damage of hsa_circ_0007919-inhibited GEM-resistant PDAC cells with or without LIG1 overexpression (200×). (N) IF analysis of γ-H2AX accumulation in hsa_circ_0007919-inhibited GEM-resistant PDAC cells with or without LIG1 overexpression (1000×). Data are the means ± SDs (n = 3 independent experiments), * p < 0.05, ** p < 0.01, *** p < 0.001

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Molecular Cancer

ISSN: 1476-4598