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Fig. 7 | Molecular Cancer

Fig. 7

From: hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma

Fig. 7

hsa_circ_0007919 recruits FOXA1 and TET1 to promote LIG1 transcription

(A) DNA methylation analysis of CpG island of LIG1 promoter. (B) Interaction region predicted between FOXA1 and LIG1 promoter. (C) Expression of LIG1 in GEM-resistant PDAC cells with or without 5-AzaC treatment. (D-E) ChIP assay analysis of the interaction between FOXA1 or TET1 and LIG1 promoter. (F-G) MS-PCR analysis of methylation level of LIG1 promoter in GEM-resistant PDAC cells with or without hsa_circ_0007919 or TET1 inhibition and with or without hsa_circ_0007919 or TET1 overexpression. (H-I) Luciferase activity analysis of LIG1 transcriptional activity in GEM-resistant PDAC cells with or without hsa_circ_0007919 or FOXA1 or TET1 inhibition and with or without hsa_circ_0007919 or FOXA1 or TET1 overexpression. Data are the means ± SDs (n = 3 independent experiments), ** p < 0.01, *** p < 0.001

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Molecular Cancer

ISSN: 1476-4598