Fig. 2

CircHERC1 promotes NCSLC cell proliferation, migration and invasion, and inhibits apoptosis. (a) EdU assay to detect the proliferation of NCI-H3255 cells stably overexpressing circHERC1. **p < 0.01. The scale bar is 25 μm. (b) EdU assay to detect the proliferation of NCI-H3255 cells with stable knockdown of circHERC1. ***p < 0.001. The scale bar is 25 μm. (c) Flow cytometry to detect apoptosis of NCI-H3255 cells stably overexpressing circHERC1. ***p < 0.001. (d) Flow cytometry to detect apoptosis of NCI-H3255 cells with stable knockdown of circHERC1. **p < 0.01. (e, g) Transwell assay to detect cell migration (e) and invasion (g) of NCI-H3255 cells stably overexpressing circHERC1. **p < 0.01, ***p < 0.001. (f, h) Transwell assay to detect cell migration (f) and invasion (h) of NCI-H3255 cells with stale knockdown of circHERC1. **p < 0.01, ***p < 0.001. (i) Representative images of tumor xenograft mouse models injected with NCI-H3255 cells with stable knockdown of circHERC1 and NCI-H3255 cells stably transfected with a scramble shRNA vector as negative control (top). The tumor volumes of mice in the sh-circHERC1 and control groups are shown(bottom). **p < 0.01. (j) Western blot analysis of the MAPK/ERK, NF-κB and PI3K/AKT pathways in NCI-H3255 cells with stable knockdown and overexpression of circHERC1