Fig. 1

CircRNAs derived from the exosomes of liver CSCs play a significant role in malignant dissemination. a and b. Western blot and Real-time PCR analysis of the expression of CD133 in CD133 positive (CD133 +) and CD133 negative (CD133-) cells. c. Transwell migration assay to assess the migration ability of HepG2 and Huh7 cells cultured with conditioned medium derived from CD133- cells, CD133 + cells, and CD133 + cells treated with GW4869. d and e. Cell viability of HepG2 and Huh7 cells cultured with conditioned medium derived from CD133- cells, CD133 + cells, and CD133 + cells treated with GW4869 using the CCK-8 assay at 0 h, 24 h, 48 h, and 72 h. f. Spheroid formation assay to evaluate the spheroid-formation ability of HepG2 and Huh7 cells treated with conditioned medium derived from CD133- cells, CD133 + cells, and CD133 + cells treated with GW4869. g. Expression levels of the top 5 differentially expressed circRNAs in 20 clinical samples of liver cancer tissues and matched paraneoplastic tissues. h and i. Expression levels of circ-ZEB1 and circ-AFAP1 in well differential and poor differential liver cancer tissues. ns: not statistically significant, *P < 0.05, **P < 0.01, ***P < 0.001