Fig. 2

Circ-ZEB1 and circ-AFAP1 are closely correlated with poor prognosis and progression of liver cancer. a. CeRNA network consisting of two circRNAs (central red nodes), miRNAs (inner blue nodes) and mRNAs (outer green nodes). b. ROC curve for model evaluation. c. Kaplan‒Meier survival curves for patients in high-risk and low-risk groups based on the mRNA risk model. d. Time-dependent ROC curve for the risk model at 1-year, 3-year, and 5-year intervals. e. Nomogram based on the 16 mRNAs in the risk model. f. Expression profiles of genes in the high-risk and low-risk groups of the TCGA database within the model. g. Comparison of tumor volume with and without overexpression of circ-ZEB1 and circ-AFAP1. h. Expression of E-cadherin, CD133, and EpCAM in tumor tissues after overexpression of circ-ZEB1 and circ-AFAP1, as detected by Western blot analysis. i. Immunohistochemical staining results for Ki-67, EpCAM, and CD133 protein levels in tumor tissues after circ-ZEB1 and circ-AFAP1 overexpression. j. Study profile. Influence of relevant circRNAs in liver CSC exosomes on the malignant biological behavior of liver cancer. ns: not statistically significant, *P < 0.05, **P < 0.01