Fig. 2

USP13 overexpression drives LUSC development in the KP mouse model. A Schematic of the experimental procedure using KP and KPU mouse model. KP or KPU mice were infected with the Adeno-CMV-Cre virus (Ad5-CMV-Cre) via intratracheal administration. B Survival analysis of KP and KPU mice upon virus infection. The p-value was calculated using a log-rank test. C Representative hematoxylin and eosin (H&E) staining of KP and KPU lung at 12 weeks post-viral infection. Dotted lines indicate tumor nodules. Scale bar = 5 mm. D Quantification of individual tumor number and area in KP and KPU mice at 10-14 weeks post-Ad5-CMV-Cre virus infection (n=8 mice/group). E Representative images for H&E, USP13, NKX2-1, SPC, SOX2, and CK5 IHC stains of the KP and KPU tumors. The red boxed areas on the left images were magnified and shown on the right. Black scale bar = 50 µm, White scale bar = 20 µm. (F) Western blot showing the expression of USP13, NKX2-1, CK7, p63, and SOX2 in KP and KPU tumors. GAPDH is a loading control. G Quantification of tumor number and area for LUAD and LUSC components in KPU mice. n=8 for each group. H The ratio of NSCLC subtypes in each KPU mouse (n=8). In (D) and (G), error bars indicate mean ± SEM. Two-tailed unpaired t-tests, *p < 0.05, **p < 0.01, ***p < 0.001