Fig. 4

MYC protein is upregulated in lung cancer by USP13. A Correlation between USP13 and MYC expression (left), USP13 and SOX2 expression (center), and MYC and SOX2 expression (right) in LUSC. Data was obtained from cBioportal (CPTAC, Cell 2021) (n=80). Axis represents the protein abundance ratio. B IHC score of USP13, MYC, and SOX2 in LUAD and LUSC patients. Data are shown as means ± SEM. **p < 0.01, and ****p < 0.0001 (unpaired two-tailed t-test). C IHC analysis of USP13 and MYC protein abundance in LUSC samples (left) (n = 130) and their correlation (right). D IHC analysis of USP13 and MYC protein abundance in LUAD samples (left) (n = 51) and their correlation (right). E Representative images for IHC staining of MYC in the KP and KPU tumors post-Ad5-CMV-Cre infection. The red boxed areas on the left images were magnified and shown on the right. Black scale bar = 50 µm, white scale bar = 20 µm. F Expression of NKX2-1, SOX2, and MYC in LUAD and LUSC components in Ad5-CMV-Cre infected KPU lungs. Scale bar = 200 µm. See also Figure S4H. G Western blot of endogenous USP13, MYC, SOX2, NKX2-1, and β-actin in KP and KPU mouse cell lines. H Western blot of USP13, MYC, and β-actin in human NSCLC cells with or without USP13 overexpression. In (A), (C), and (D), data are analyzed using the Pearson correlation coefficient