Fig. 6

LUSC originates from CC10⁺ club cells in the KPU model. A Schematic of two origins of lung adenocarcinoma. Cre recombinase expression was restricted to specific cell types in the adult lung by cell type-restricted Ad-Cre viruses; Cre recombinase is expressed in alveolar type II cells (orange) and club cells (blue) by Ad5-SPC-Cre and Ad5-CC10-Cre, respectively. Bronchioalveolar stem cells (BASCs, yellow) can express Cre recombinase by both viruses B Schematic of the experimental procedure using Ad5-SPC-Cre and Ad5-CC10-Cre viruses. KPU mice were infected with cell-type restricted Ad-Cre viruses via intratracheal administration. C Quantification of individual tumor number (left) and area (right) in KPU mice at 12-14 weeks post-virus infection (n=7 mice/group). D Representative images of H&E, NKX2-1, and SOX2 staining from lung tumors with indicated Cre infection. Scale bar = 25 µm E Quantification of proportion (top) and size (bottom) of lesions as indicated in D F Expression of NKX2-1 and SOX2 during cancer development in KPU mice infected with Ad5-SPC-Cre or Ad5-CC10-Cre. Arrow and dotted lines indicate hyperplasia and carcinoma in situ (CIS), respectively. Scale bar = 50 µm G IHC quantification for NKX2-1 (top) and SOX2 (bottom). Each dot represents one tumor nodule from seven mice per group. a.u., arbitrary unit. In C, E, and G, error bars indicate mean ± SEM. Two-tailed unpaired t-tests, ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001