Fig. 2

Establishment of patient-derived Malignant peritoneal mesothelioma (MPM) organoid cultures. A Representative images of MPMOs culture process, including the generation of MPM primary cells. B The PET/CT images of MPM patients showed significantly enhanced dense shadows in multiple areas of the abdominal cavity, and there were radioactive concentrations of imaging agents, indicating that the patient’s abdominal cavity has been invaded by multiple tumors. C Heat map showing the fraction of the derived organoids in the D1 and D7. D Expansion potential of MPM- and CPM-derived organoid. Arrow, continuous expansion ; Dot, passage ; Cross, Stop growth/death; MPM, malignant peritoneal mesothelioma ; CPM, cystic peritoneal mesothelioma ; O, organoid. E Representative bright field images of MPM and CPM-derived organoids from 4 patients. Scale, 50 μm