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Fig. 3 | Molecular Cancer

Fig. 3

From: Organoids derived from patients provide a new opportunity for research and individualized treatment of malignant peritoneal mesothelioma

Fig. 3

Culture characteristics of MPMOS and composition of culture medium. (A) MPMOs formed in different tumor-organoid culture medium. The image shown is from MPM-C1-O as a representative sample after 10 days of planting. Left, bright field images, scale, 200 μm;Right, Quantification of the MPMOs number per well in the different tumor-organoid culture medium. (B) The formation of MPM organoids culture in factor deletion medium after 10 days. The images shown are from MPM-C2-O as a representative sample. Left, bright field images, scale, 200 μm;Right, Quantification of the MPMOs number per well. (C) Time-lapse photography of images with different magnification of MPMOs. Left, bright field images, Scale bar, 400 μm, 200 μm, 100 μm, 50 μm;Right, Quantification of the MPMOs number per well for individual organoid. Data were showed as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 and each experiments were performed in triplicate

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