Fig. 8

Cytological characteristics of malignant peritoneal mesothelioma (MPM) primary cell lines. A Morphology was showed under the inverted phase contrast microscope of MPM-C6 primary cell. Scale bar, 100 μm. B The MPM-C6 primary cell morphology was revealed by phalloidin staining. Scale bar, 10 μm. C Multiple immuoflurescence staining of cytokeratin 5/6 (CK5/6), Wilms Tumor (WT-1), calretinin on MPM-C6 primary cells. CK5/6, red;WT-1, green;Calretinin, pink. Scale bar, 10 μm. D The genomic authentication of the MPM-C6 primary cell was confirmed by the short tandem repeats (STRs). E Heat map of logIC50 values for 14 anticancer drugs used to treat MPM-C6 primary cell and related MPM organoids. Putative targets of the tested anticancer drugs are listed on the left. F Representative dose response curves of the MPM-C6 primary cell and related MPM were treated with first-line chemotherapy drugs alone. Data were showed as mean ± SD and each experiment were performed in triplicate