Fig. 9
Drug response in malignant peritoneal mesothelioma (MPM) organoid lines. A Heat map of logIC50 values of 14 anticancer drugs used to treat seven MPMOs and a CPMO from 8 patients. All organoids are of the first generation. B Dose response curves of first-line chemotherapy drugs for MPMOs and CPMO. C MPM organoids morphology after first-line chemotherapy drugs treated were showed under the inverted phase contrast microscope. The image shown are from MPM-C7-O (drug resistance) and MPM-C6-O (drug sensitivity) as a representative sample. D CT scan images show the tumor status of MPM patients during treatment. Primary tumor (circled in cyan), metastatic lesion (circled in red), ascites (circled in yellow). E Fitted dose–response curves illustrating the distinct responses of MPMOs to pemetrexed and cisplatin. F The serum CA125 levels in different MPM patients pre and post treatment. G The overall survival of the MPM and CPM patients in this study. Data were showed as mean ± SD and each experiment were performed in triplicate