Fig. 2

Identification and edition of gene regulatory elements at chr11q22.2. a. Scheme of identified enhancer and insulator elements. CRISPR/Cas9-disrupted Insulator element 8 (IE8) is highlighted. b. Expression profile of 5’ and 3‘MMP genes in TNBC cell lines. c. Hi-C contact frequency matrix for the 3 Mb genomic region surrounding IE8 binned at 10-kb resolution. d. CTCF binding is represented as fold enrichment (top) relative to isotype control in MDA-MB-231 and MDA-MB-436 and (bottom) relative fold enrichment after IE8 disruption. Student’s T-test. *P < 0.05, **P < 0.01