Fig. 4

mRNA expression changes after IE8 disruption. a. Volcano plot summarizes the RNA-seq results in MDA-MB-231. 166 genes were significantly upregulated (Padj < 0.05) (blue dots), whereas only 71 were downregulated (red dots). b. Representation factor of differentially expressed genes in MDA-MB-231. Hypergeometric test. ns P > 0.05. c. Variation of MMP8 and MMP1 RNA expression in MDA-MB-231 in RNA-seq. Student’s T-test. *P < 0.05, **P < 0.01. d. MMP1 (top) and MMP8 (bottom) mRNA expression levels determined by qPCR in MDA-MB-231 and MDA-MB-436 models. Student’s T-test. ns P > 0.05, *P < 0.05, **P < 0.01 e. Variation of accessibility in enhancers close to MMP2 (top) and MMP9 (bottom) promoters. f. MMP2 (top) and MMP9 (bottom) mRNA expression levels determined by qPCR in MDA-MB-231 and MDA-MB-436 models. Student’s T-test. ns P > 0.05, *P < 0.05, **P < 0.01