Fig. 2

Combination therapy with doxorubicin leads to complete tumor cure. A Schematic representation of the experimental setup. B, E B16 melanoma cells were s.c. inoculated. Results show a summary of three independent experiments (6–8/group/experiment). C, F 4T1 mammary carcinoma cells were s.c. inoculated. Shown is one experiment (n = 6). D, G Orthotopic 4T1 mammary carcinoma model. Results show a summary of two independent experiments (6/group/experiment). Tumor growth progression is depicted for the individual mice in each group (B-D). Kaplan Meier graphs (E–G) show tumor free mice. When palpable tumors were detected, mice were treated with PBS (grey), doxo (black), 30 μg of Bisp-AFN Clec9A-PDL1-AFN (green) or a combination of Clec9A-PDL1-AFN with doxo (blue). Black lines underneath the X-axis depict the treatment time, while orange arrows indicate s.c. administration of doxo. Kaplan Meier plots depicting % tumor free mice (E–G) were analyzed using log-rank (Mantel-Cox) test with * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001