Fig. 4
Induction of immunological memory upon combined treatment with doxorubicin. A Schematic representation of the experimental layout. Tumor-free mice over the different experiments were re-challenged s.c. in the contralateral flank on day 25–30 after eradication of the primary tumor. For each tumor model, mice without prior tumor inoculation nor treatment, referred to as ‘naive mice’, were included and injected with the indicated tumor cells as a control for disease progression. B-E Mice cured from an s.c. primary tumor were s.c. re-challenged with 6 × 105 B16 cells (B-C) or 105 4T1 cells (D-E). F-G Mice in which the orthotopically implanted primary 4T1 tumor was completely cured were re-challenged s.c. with 105 4T1 cells. Figures show tumor growth measured of individual mice in each group (B, D, F) as well as % tumor free mice (C, E, G). Kaplan Meier graphs depicting % tumor free mice (C, E, G) were analyzed using log-rank (Mantel-Cox) test. * < 0.05; ** < 0.01; *** < 0.001; **** < 0.0001