Fig. 6

scRNAseq reveals key shifts in both lymphoid and myeloid cells in the tumor microenvironment. A Schematic representation of the scRNA sequencing experiment. Data obtained upon administration of PBS are represented in grey while data obtained after Clec9A-PDL1-AFN administration are visualized in green. B, C UMAP visualization showing the overall CD45⁺ living cell population in B16 tumors (B) as well as for the individual treatment groups (C). D Annotation plot showing differentially expressed (DE) genes in the X-axis to determine the different clusters (Y-axis). The size of the dot indicates the number of cells that express the gene of interest. The color intensity reflects the expression level (red = high, blue = low). E–G Parts of whole graphs showing relative distribution of immune cell types over the different treatments