Fig. 8

Bisp-AFN induces migration and potent maturation of dendritic cells. A Schematic representation of the experiment. Data obtained upon administration of PBS are represented in grey while data obtained after Clec9A-PDL1-AFN administration are visualized in green. B-D Presence and maturation status as determined by CD40 expression of cDC1 in B16 tumor (B) as well as migratory cDC1 (C) and resident cDC1 (D) in draining LN. cDCs were determined as CD45⁺ living cells, CD11c⁺MHC-II⁺. LN migratory cDCs are CD11c^intermediateMHC-II^high while LN resident cDCs are CD11c^highMHC-II^intermediate. cDC1 were determined as CD11b⁻XCR1.⁺ cells within the described cDC population. Results show bar charts of individual values (B, C, D) with mean ± SEM. Graphs were analyzed using unpaired nonparametric Mann–Whitney t-test (migratory cDC1 within draining LN) or unpaired parametric t-test. * < 0.05; ** < 0.01; *** < 0.001; **** < 0.0001