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Fig. 2 | Molecular Cancer

Fig. 2

From: CircPPAP2B controls metastasis of clear cell renal cell carcinoma via HNRNPC-dependent alternative splicing and targeting the miR-182-5p/CYP1B1 axis

Fig. 2

CircPPAP2B promotes ccRCC proliferation and metastasis in vivo and in vitro A-C Colony formation assays, CCK8 assays, and Edu assays were performed to evaluate the proliferation ability of ccRCC cells treated with shNC or shcircPPAP2B. D-E Transwell assays and wound-healing assays were conducted to detect the invasive ability of ccRCC cells treated with shNC or shcircPPAP2B. F The representative images of nude mice subcutaneously injected with caki-1 stably expressed shNC or shcircPPAP2B. G The volume of tumors was measured every week. H The weight of tumors was measured 5 weeks after injection. I The representative images of lung metastases in nude mice by HE staining. Tumor burdens were reflected by the percentages of tumor area in each slide. J Transwell assays were conducted to detect the invasive ability of ccRCC cells treated with OE-NC or OE-circPPAP2B. K-L CCK8 assays and Edu assays were performed to evaluate the proliferation ability of ccRCC cells treated with OE-NC or OE-circPPAP2B. Data are represented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. WT group

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Molecular Cancer

ISSN: 1476-4598