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Fig. 4 | Molecular Cancer

Fig. 4

From: CircPPAP2B controls metastasis of clear cell renal cell carcinoma via HNRNPC-dependent alternative splicing and targeting the miR-182-5p/CYP1B1 axis

Fig. 4

CircPPAP2B facilitates HNRNPC nuclear translocation via regulating HNRNPC nondegradable ubiquitination and stabilizing HNRNPC/Vimentin/Importin α7 ternary complex A RNA FISH was performed to determine subcellular localization of HNRNPC in highly and poorly invasive ccRCC cell lines. B CoIP assay was performed to determine the direct binding between HNRNPC with Vimentin and Importin α. C Endogenous CoIP assay was performed to confirm the direct interaction between HNRNPC and Vimentin with Importin α7, and the role of the m6A modification site in their interaction. D CoIP assay was performed to explore whether circPPAP2B regulates the ubiquitination of HNRNPC in ccRCC cell lines. E CoIP assay was performed to determine whether the regulatory of circPPAP2B on HNRNPC ubiquitination is dependent on m6A modification. F CoIP assay was performed to confirm the direct interaction between HNRNPC and TRIM25 or USP10. G CoIP assay was performed to determine the role of USP10 on ubiquitination levels of HNRNPC. H CoIP assay was performed to explore whether HNRNPC ubiquitination regulates the interaction between HNRNPC and Importin α7. I FISH was performed to determine the role of TRIM25 or USP10 on the subcellular localization of HNRNPC in ccRCC cells

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Molecular Cancer

ISSN: 1476-4598