Fig. 1
From: Stratifying esophago-gastric cancer treatment using a patient-derived organoid-based threshold
Trial flow chart and phenotypic, molecular as well as functional characterization of PDO cohort. A Flow chart of the study population. DD/HD: University hospital of Dresden (DD) and Heidelberg (HD). B Brightfield images of a representative EGC organoids line (OO4) and a normal gastric PDO line as a comparison (scale bar: 50 µm). Representative images of the histopathological characterization of primary tumor and corresponding PDO (OO4). The treatment naïve tumor biopsy, thereof derived organoid culture and the post-treatment resection specimen were characterized by hematoxylin and eosin (HE) staining, carcinoembryonic antigen (CEA) staining, periodic acid-Schiff reaction (PAS) as well as cytokeratin7 and cadherin17 (CK7/CADH17) double-staining (scale bar: 50 μm). C Oncoplot depicting prevalent genetic alterations in primary tumors and derived PDO lines. D Bar graph of mutational frequencies of this study. E Dose response curves from cell viability assay of 5-FU treated PDOs 144 h post treatment (average of n = 3 replicates per PDO). F Combined dose response curves from PDOs according to the patients' pathological response of 5-FU with standard deviations (repeated measures analysis of variance (ANOVA) of grouped PDOs, P = 0.001 for 5-FU). *P < 0.05; **P < 0.01. G Comparison of the relative area under the curve (AUCrel) of PDOs grouped by the patients' pathological response (R: responder; NR: non-responder) for 5-FU (unpaired two-tailed student's t-test, P = 0.010 for 5-FU, *P < 0.05). Blue lines represent pathological non-responders, black lines are pathological responders