Fig. 2
From: Stratifying esophago-gastric cancer treatment using a patient-derived organoid-based threshold
Establishment and validation of an in vitro threshold value to predict in vivo FLOT response. A Dose response curves (DRC) from cell viability assay of PDOs treated with the standard FLOT mixture (n) in varying dilutions analyzed 144 h post treatment (average of n = 3 replicates per PDO). B Combined FLOT-DRC from PDOs according to the patients' pathological response after FLOT neoCTx with standard deviations (repeated measures analysis of variance (ANOVA) of grouped PDOs, P = 4.55e-06). ***P < 0.001 C, Comparison of the relative area under the curve (AUCrel) of PDOs grouped by the patients' pathological response (R: responder; NR: non-responder) (two-tailed student's t-test, P = 3.94e-05). ***P < 0.001 D Dot plot of AUCrel(FLOT) ordered by the patients’ pathological response. Correlation analysis was performed using Kendall ordinal correlation. P < 0.005 was considered statistically significant. E Heatmap of the patients' pathological response and AUCrel derived Z scores from single drug and FLOT combination treatment. Color scale indicates Z score (± 2.5; -2.5: black, 0: white, + 2.5: blue) and scored patients' pathological response according to Becker et al. F ROC curve of the AUCrel(FLOT) generated from single FLOT treatments of PDOs (n = 39, 3 replicates of 13 PDOs). G Summary graph of the AUCrel(ROC) and confidence interval (CI) 95% for FLOT, 5-FU, oxaliplatin and docetaxel. H Dose response curve with CI of FLOT combination treatment grouped according to the patients' pathological response with calculated threshold curve and calculated AUCthreshold(FLOT). Blue lines represent pathological non-responders, black lines are pathological responders. I FLOT combination test results with subsequent classification according to the determined threshold value (AUCthreshold = 0.559) into responding (R) and non-responding (NR) patients. Correctness of classification was later assessed by comparison to patients' pathological response. Red dotted line indicates the threshold established in the exploratory cohort. J Achieved sensitivity, specificity and accuracy depending on the number of performed FLOT testings considered for classification. K ROC curve of the AUCrel(FLOT) generated from the mean of three independent replicates for each PDO of the whole cohort (n = 26)