Fig. 5

circCGNL1 enhances HDAC4 dephosphorylation by interacting with NUDT4. A, B We overexpressed or knocked down circCGNL1 in PANC-1 cells and performed Co-IP assays with NUDT4 or HDAC4 as bait proteins. Immunoblotting showed that the combination of NUDT4 and HDAC4 was increased by circCGNL1. C WB analysis indicated that HDAC4 serine phosphorylation decreased significantly following circCGNL1 overexpression. D Rescue experiments showed that silencing NUDT4 reversed the dephosphorylation effect of circCGNL1 on HDAC4 (S632). E–G The cytoplasmic and nuclear HDAC4-expression levels in PC cells transfected with pcDNA3.1 (empty vector) or pcDNA3.1-circCGNL1 were detected by WB (E) and IF assays (F, G). Relative fluorescence intensity of nucleus HDAC4 was the fluorescence intensity of nuclear HDAC4 in circCGNL1 overexpressing group versus that in empty vector group. The fluorescence intensity of Nucleus HDAC4 was measured by ImageJ. ns: no significance, *p < 0.05, **p < 0.01