Fig. 8

RUNX2 promotes PC progression by repressing GAMT. A Proliferation of PANC-1 and BxPC-3 cells transected with an RUNX2-overexpression vector, co-transfected with RUNX2-GAMT overexpression vectors, or the pcDNA3.1 vector control was studied using EdU assays. B TUNEL assays were used to detect PC cells apoptotic rate. Gemcitabine (HY-17026, MCE) was used to treat PANC-1 and BxPC-3 cells at 1 μmol/L for 24 h to induce cell apoptosis. C DAPI staining assay were applied to detect PC cell apoptosis. Cell fluorescence intensity was measured by ImageJ. PC cell nuclear chromatin morphological changes were observed by TEM. The red arrows showed the apoptotic bodies. D Expression levels of proteins related to apoptosis. Protein extracts from pcDNA3.1-GAMT and pcDNA3.1 transfectants analyzed using WB assay to detect total and phosphorylated AMPK, AKT, Bad, and cleaved caspase-3. ns: no significance, *p < 0.05, **p < 0.01